TBP Polyclonal Antibody (OABB00488)

Catalog No: OABB00488 (Formerly GWB-BBP411)

Size:100UG

Lane 1: MCF-7 Cell Lysate
Lane 2: M231 Cell Lysate
Lane 3: M231 Cell Lysate

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: TBP, TATA-binding protein, is a general transcription factor that binds specifically to a DNA sequence called the TATA box. The TBP gene is mapped to 6q27. This DNA sequence is found about 35 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitiation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II.
  Background: TBP, TATA-binding protein, is a general transcription factor that binds specifically to a DNA sequence called the TATA box. The TBP gene is mapped to 6q27. This DNA sequence is found about 35 base pairs upstream of the transcription start site in some eukaryotic gene promoters.[1] TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex.[2] As one of the few proteins in the preinitiation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human: Mouse, Rat
• Reference: 1. Bauer, P., Laccone, F., Rolfs, A., Wullner, U., Bosch, S., Peters, H., Liebscher, S., Scheible, M.,
  Epplen, J. T., Weber, B. H. F., Holinski-Feder, E., Weirich-Schwaiger, H., Morris-Rosendahl, D. J.,
  Andrich, J., Riess, O.Trinucleotide repeat expansion in SCA17/TBP in white patients with
  Huntington's disease-like phenotype.J. Med. Genet. 41: 230-232, 2004.
  2. Stevanin, G., Fujigasaki, H., Lebre, A.-S., Camuzat, A., Jeannequin, C., Dode, C., Takahashi, J.,
  San, C., Bellance, R., Brice, A., Durr, A.Huntington's disease-like phenotype due to trinucleotide
  repeat expansions in the TBP and JPH3 genes.Brain 126: 1599-1603, 2003.
  3. Veenstra, G. J. C., Weeks, D. L., Wolffe, A. P.Distinct roles for TBP and TBP-like factor in early
  embryonic gene transcription in Xenopus.Science 290: 2312-2314, 2000.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for TATA-box-binding protein(TBP) detection. Tested with WB in Human;Mouse;Rat.

Target Info

• Gene Symbol: TBP
• Gene Full Name: TATA-box binding protein
• Alias Symbols: GTF2D;GTF2D1;HDL4;SCA17;TATA sequence-binding protein;TATA-binding factor;TATA-box binding protein N-terminal domain;TATA-box factor;TATA-box-binding protein;TFIID;transcription initiation factor TFIID TBP subunit.
• NCBI Gene Id: 6908
• Protein Name: TATA-box-binding protein
• Description of Target: General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription (PubMed:15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
• Uniprot ID: P20226
• Molecular Weight: 37698 MW