TAF15 Antibody (OAGA02076)

Catalog No: OAGA02076

Size:100 ul

Sample (30 ug of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
OAGA02076 diluted at 1:1000

Sample (30 ug whole cell lysate)
A:Raji
7.5% SDS PAGE
OAGA02076 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using TAFII68 (OAGA02076) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAFII68 (OAGA02076) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TAFII68. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: TAF15
• Gene Full Name: TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
• Alias Symbols: Npl3, RBP56, TAF2N, TAFII68
• NCBI Gene Id: 8148
• Description of Target: This gene encodes a member of the TET family of RNA-binding proteins. The encoded protein plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Uniprot ID: Q92804