STAT6 ELISA Kit (Human) (OKEH07175)

Catalog No: OKEH07175

Size:96T

Human STAT6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology STAT6 ELISA Kit (Human) (OKEH07175) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-STAT6 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated STAT6 and incubated. The STAT6 found in the sample or standards competes with the biotinylated STAT6 for limited binding sites on the immobilized anti-STAT6 antibody. Excess unbound biotinylated STAT6 and sample or standard STAT6 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated STAT6 captured in the well and inversely proportional to the amount of STAT6 which was contained in the sample or standard.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into matrix = 109.0%
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
  Intra-Assay CV <7.9%
  Inter-Assay CV <10.3%
• ELISA Kit Component:
  • STAT6 Microplate: 96 Wells (12 x 8 Well strips)
  • STAT6 Lyophilized Standard: 2 x 100 ng
  • 100X STAT6 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.811 ng/mL
• Specificity: Natural and recombinant Human Signal transducer and activator of transcription 6
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: STAT6
• Gene Full Name: signal transducer and activator of transcription 6
• Alias Symbols: D12S1644;IL-4 Stat;IL-4-STAT;signal transducer and activator of transcription 6;signal transducer and activator of transcription 6, interleukin-4 induced;STAT, interleukin4-induced;STAT6B;STAT6C;transcription factor IL-4 STAT.
• NCBI Gene Id: 6778
• Protein Name: P42226
• Description of Target: Signal transducer and activator of transcription 6
• Uniprot ID: https://www.uniprot.org/uniprot/P42226
• Protein Accession #: NP_001171549
• Nucleotide Accession #: NM_001178078