SMC1A Antibody - C-terminal region (ARP38780_P050)

Catalog No: ARP38780_P050

WB Suggested Anti-SMC1A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 293T cell lysateSMC1A is supported by BioGPS gene expression data to be expressed in HEK293T

Host: Rabbit
Target Name: SMC1A
Sample Type: Human HepG2
Antibody Dilution: 1.0ug/mlSMC1A is supported by BioGPS gene expression data to be expressed in HepG2

Host: Rabbit
Target Name: SMC1A
Sample Type: Human Jurkat
Antibody Dilution: 1.0ug/mlSMC1A is supported by BioGPS gene expression data to be expressed in Jurkat

Rabbit Anti-SMC1A Antibody
Catalog Number: ARP38780_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SMC1A
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 77%
• Peptide Sequence: Synthetic peptide located within the following region: VISLKEEFYTKAESLIGVYPEQGDCVISKVLTFDLTKYPDANPNPNEQ
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-SMC1A (ARP38780_P050) antibody is Catalog # AAP38780 (Previous Catalog # AAPP20985)
• Sample Type Confirmation:

  SMC1A is supported by BioGPS gene expression data to be expressed in HEK293T, HepG2, Jurkat

• Reference: Barber,T.D., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (9), 3443-3448

Target Info

• Gene Symbol: SMC1A
• Gene Full Name: Structural maintenance of chromosomes 1A
• Alias Symbols: SMC1, SMCB, CDLS2, DEE85, SB1.8, EIEE85, SMC1L1, DXS423E, SMC1alpha
• NCBI Gene Id: 8243
• Protein Name: Structural maintenance of chromosomes protein 1A
• Description of Target: The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by SMC1A gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the SMC1A protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: Q14683
• Protein Accession #: NP_006297
• Nucleotide Accession #: NM_006306
• Protein Size (# AA): 1233
• Molecular Weight: 143kDa
• Protein Interactions: UBC; SUMO2; STAU1; RPA3; RPA2; RPA1; RNF2; EED; KIF13A; ARMCX3; CDK20; ANKRD28; HDAC11; HDAC8; SMC1A; RFC1; POLA1; MCM7; MCM6; METTL21B; WHSC1; NOTCH1; SF3B3; ACIN1; SMC2; RBM14; SMC3; RAD21; NSMCE2; FBXO6; CUL3; NEDD8; CDK2; CEBPA; CDK8; NIPBL; MDC1; POL