SMC1A Antibody - N-terminal region (OAAB22138)

Catalog No: OAAB22138

Size:100ul

Western blot detection of SMC1A (N-terminus) in HelaNE, Jurkat and A431 cell lysates using SMC1A - N-Terminus Antibody (OAAB22138) (1:1000 diluted). Predicted band size: 143KDa. Observed band size: 143KDa.

IHC of paraffin-embedded human colon using SMC1A - N-Terminus Antibody (OAAB22138) diluted 1/500-1/1000.

Flow Cytometry analysis of HeLa cells stained with SMC1A (N-terminus) (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Function: Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Formulation: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
• Application Info: WB: 1:1000
  IHC: 1:500
  FC: 1:100
• Reference: Rocques P.J.,et al.Hum. Mol. Genet. 4:243-249(1995).
  Nagase T.,et al.DNA Res. 3:17-24(1996).
  Nakajima D.,et al.DNA Res. 9:99-106(2002).
  Ross M.T.,et al.Nature 434:325-337(2005).
  Yazdi P.T.,et al.Genes Dev. 16:571-582(2002).
  

Target Info

• Gene Symbol: SMC1A
• Gene Full Name: structural maintenance of chromosomes 1A
• Alias Symbols: SMC1, SMCB, CDLS2, DEE85, SB1.8, EIEE85, SMC1L1, DXS423E, SMC1alpha
• NCBI Gene Id: 8243
• Description of Target: Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Uniprot ID: Q14683
• Protein Accession #: NP_006297.2
• Nucleotide Accession #: NM_006306.3
• Molecular Weight: 143 kDa