Catalog No: OKCD08631
Size:96WELLS
Price: $825.00
SKU
OKCD08631
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for SLC1A2 ELISA Kit (Mouse) (OKCD08631) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excitatory Amino Acid Transporter 2 (EAAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Excitatory Amino Acid Transporter 2 (EAAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Excitatory Amino Acid Transporter 2 (EAAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Excitatory Amino Acid Transporter 2 (EAAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 2 (EAAT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Excitatory Amino Acid Transporter 2 (EAAT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.054ng/mL |
Gene Symbol | Slc1a2 |
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Gene Full Name | solute carrier family 1 (glial high affinity glutamate transporter), member 2 |
Alias Symbols | 1700091C19Rik;2900019G14Rik;AI159670;Eaa;Eaat2;EAAT2 variant mEAAT2/5UT2;EAAT2 variant mEAAT2/5UT5;excitatory amino acid transporter 2;GLT;GLT-;GLT1;GLT-1;glutamate transporter 1c;MGLT;MGLT1;sodium-dependent glutamate/aspartate transporter 2;Solute carrier family 1 member 2;solute carrier family 1, member 2. |
NCBI Gene Id | 20511 |
Protein Name | Excitatory amino acid transporter 2 |
Description of Target | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7698742, PubMed:7557442, PubMed:9373176). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (PubMed:9180080). |
Uniprot ID | P43006 |
Protein Accession # | NP_001070982.1 |
Nucleotide Accession # | NM_001077514.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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