SELENBP1 ELISA Kit (Human) (OKCD08914)

Catalog No: OKCD08914

Size:96WELLS

SELENBP1 ELISA Kit (Human) (OKCD08914) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selenium Binding Protein 1 (SELENBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Selenium Binding Protein 1 (SELENBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Selenium Binding Protein 1 (SELENBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Selenium Binding Protein 1 (SELENBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Selenium Binding Protein 1 (SELENBP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Selenium Binding Protein 1 (SELENBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • SELENBP1 Microplate: 96 Wells (12 x 8 Well strips)
  • SELENBP1 Lyophilized Standard: 2
  • 100X SELENBP1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.055ng/mL

Target Info

• Gene Symbol: SELENBP1
• Gene Full Name: selenium binding protein 1
• Alias Symbols: 56 kDa selenium-binding protein;EHMTO;epididymis secretory sperm binding protein Li 134P;HEL-S-134P;hSBP;LPSB;methanethiol oxidase;MTO;SBP56;Selenium-binding protein 1;SP56.
• NCBI Gene Id: 8991
• Protein Name: Methanethiol oxidase
• Description of Target: Catalyzes the oxidation of methanethiol, an organosulfur compound known to be produced in substantial amounts by gut bacteria (PubMed:29255262). Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport (By similarity).
• Uniprot ID: Q13228
• Protein Accession #: NP_001245217.1
• Nucleotide Accession #: NM_001258288.1