S1P1 Antibody (OAPB00756)

Catalog No: OAPB00756

Size:100UG

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Western blot analysis of S1P1 in mouse thymus lysate with S1P1 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry of S1P1 in mouse thymus tissue with S1P1 antibody at 5 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Liquid. S1P1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: S1P1 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: S1P1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human S1P1. The immunogen is located within the last 50 amino acids of S1P1.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-S1P1 antibody is Catalog # OEPB02879
• Sample Type Confirmation: Mouse Thymus Tissue Lysate
• Specificity: At least two isoforms of S1P1 are known to exist; this S1P1 antibody will only recognize the shorter isoform.
• Dilution: Optimal dilutions for each application to be determined by the researcher.
• Intended Use: FOR RESEARCH USE ONLY
• Application Info: Western blot: 1 - 2 μg/mL
  Immunohistochemistry: 2.5 μg/mL
• Reference: Miyasaka M and Tanaka T. Lymphocyte tracking across high endothelial venules: dogmas and enigmas. Nat. Rev. Immunol.2004; 3:360-70.;Mandala S, Hajdu R, Bergstrom J, et al. Alteration of lymphocyte tracking by sphingosine-1-phosphate receptor agonists. Science2002; 296:346-9.;Ledgerwood LG, Lal G, Zhang N, et al. The sphingosine-1-phosphate receptor 1 causes tissue retention by inhibiting the entry of peripheral tissue T lymphocytes into afferent lymphatics. Nat. Immunol.2008; 9:42-53.;Pham TH, Okada T, Matloubian M, et al. S1P1 receptor signaling overrides retention mediated by G alpha i-coupled receptors to promote T cell egress. Immunity2008; 28:122-33.

Target Info

• Gene Symbol: S1PR1
• Gene Full Name: sphingosine-1-phosphate receptor 1
• Alias Symbols: CD363;CHEDG1;D1S3362;ECGF1;EDG1;EDG-1;endothelial differentiation G-protein coupled receptor 1;endothelial differentiation, sphingolipid G-protein-coupled receptor, 1;S1P receptor 1;S1P receptor Edg-1;S1P1;sphingosine 1-phosphate receptor 1;sphingosine 1-phosphate receptor Edg-1;sphingosine 1-phosphate receptor EDG1.
• NCBI Gene Id: 1901
• Protein Name: Sphingosine 1-phosphate receptor 1|sphingosine 1-phosphate receptor 1 [Homo sapiens]
• Description of Target: G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis (By similarity). Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury.
• Uniprot ID: P21453
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001391
• Molecular Weight: Predicted: 42 kDa Observed: 42 kDa