S1PR1 Antibody (Phospho-Thr236) (OASG02307)

Catalog No: OASG02307

Size:100 uL

Western Blot using HEPG2-UV, Mouse Brain

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF
• Additional Information: Function: G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis . Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury.
  Cellular Localization: Cell membrane. Multi-pass membrane protein. Endosome. Membrane raft. Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization.
  Tissue Specificity: Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
• Reconstitution and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived between 180-260 aa from human EDG-1 around the phosphorylation site of T236.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.
• Specificity: Phospho-EDG-1 (T236) Polyclonal Antibody detects endogenous levels of EDG-1 protein only when phosphorylated at T236.
• Application Info: IF: 1:200-1:1000
  ELISA: 1:10000
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: S1PR1
• Gene Full Name: sphingosine-1-phosphate receptor 1
• Alias Symbols: EDG1, S1P1, CD363, ECGF1, EDG-1, CHEDG1, D1S3362
• NCBI Gene Id: 1901; 13609; 29733
• Uniprot ID: P21453, O08530, P48303
• Molecular Weight: 43 kDa