RUNX2 ELISA Kit (Mouse) (OKEH07140)

Catalog No: OKEH07140

Size:96T

Mouse RUNX2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Runx2 ELISA Kit (Mouse) (OKEH07140) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Runx2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Runx2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Runx2 captured in the well.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into matrix = 96.5%
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
  Intra-Assay CV <6.4%
  Inter-Assay CV <8.7%
• ELISA Kit Component:
  • Runx2 Microplate: 96 Wells (12 x 8 Well strips)
  • Runx2 Lyophilized Standard: 2 x 40 ng
  • 100X Runx2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.32 ng/mL
• Specificity: Natural and recombinant Mouse Runt-related transcription factor 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Runx2
• Gene Full Name: runt related transcription factor 2
• Alias Symbols: acute myeloid leukemia 3 protein;AKV core binding factor;AM;AML3;Cbf;Cbfa;Cbfa1;Cbfa-1;CBF-alpha-1;core binding factor alpha 1;Core-binding factor subunit alpha-1;LS3;Oncogene AML-3;Os;Osf2;osteoblast-specific transcription factor 2;PEA2-alpha A;PEB;PEBP2 alpha A;Pebp2a1;PEBP2aA;Pebpa2a;polyomavirus enhancer-binding protein 2 alpha A subunit;runt domain, alpha subunit 1;runt-related transcription factor 2;SL3/AKV core-binding factor alpha A subunit;SL3-3 enhancer factor 1 alpha A subunit;transcription factor Cbfa1/Osf2.
• NCBI Gene Id: 12393
• Protein Name: Q08775
• Description of Target: Runt-related transcription factor 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q08775
• Protein Accession #: NP_001139510
• Nucleotide Accession #: NM_001146038