RSPO2 ELISA Kit (Mouse) (OKCA02189)

Catalog No: OKCA02189

Size:96 Wells

Mouse RSPO2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	97	92-102
  Serum (n=4)	1:2	91	85-98
  Serum (n=4)	1:4	90	88-93
  Serum (n=4)	1:8	100	96-104

• ELISA Kit Principle: Aviva Systems Biology Rspo2 ELISA Kit (Mouse) (OKCA02189) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RSPO2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RSPO2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample RSPO2 captured in well.
• ELISA Kit Range: 31.25 pg/mL-2,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	90	87-92
  EDTA plasma (n=4)	106	103-110

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-RSPO2 Microplate: 96 Wells (12 x 8 Well strips)
  • RSPO2 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated RSPO2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 7.8 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RSPO2
• Alias Symbols: ft, ftls, AA673245, D430027K22, 2610028F08Rik
• NCBI Gene Id: 239405
• Protein Name: R-spondin-2
• Description of Target: Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors.
• Uniprot ID: Q8BFU0
• Protein Accession #: NP_766403
• Nucleotide Accession #: NM_172815