PTK2 Antibody (Phospho-Tyr397) (OABF01175)

Catalog No: OABF01175

Size:100UL

L1 mouse intestine lysate L2 rat lung lysates probed with Anti Phospho-FAK (Tyr397) Polyclonal Antibody, Unconjugated (OABF01175) at 1:200 overnight at 4C. Followed by conjugation to secondary antibody at 1:3000 for 90 min at 37C. Predicted band 119kD. Observed band size: 55kD, 60kD, and 119kD

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-GnRHR Polyclonal Antibody, Unconjugated (OABF01175) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Product Info

• Predicted Species Reactivity: Bovine|Chicken|Dog|Horse|Human|Mouse|Rabbit|Rat
• Product Format: Liquid. Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Subcellular location: Cytoplasm, Nucleus, Cell membrane
  Modification: Phosphorylation (Tyr397)
• Reconstitution and Storage: -20°C
• Immunogen: KLH cunjugated synthetic phosphopeptide derived from human FAK 1 around the phosphorylation site of Tyr397
• Purification: Protein A Purified
• Concentration: 1 ug/ul
• Application Info: WB: 1:100 - 1000
  IHC-P: : 1:100 - 500

Target Info

• Gene Symbol: PTK2
• Gene Full Name: protein tyrosine kinase 2
• Alias Symbols: FADK;FADK 1;FAK;FAK1;FAK-related non-kinase polypeptide;focal adhesion kinase 1;focal adhesion kinase-related nonkinase;FRNK;p125FAK;pp125FAK;PPP1R71;protein phosphatase 1 regulatory subunit 71;Protein-tyrosine kinase 2;PTK2 protein tyrosine kinase 2.
• NCBI Gene Id: 5747
• Protein Name: Focal adhesion kinase 1
• Description of Target: Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
• Uniprot ID: Q05397