Psen1 ELISA Kit (Mouse) (OKCD01650)

Catalog No: OKCD01650 (Formerly GWB-KBBYG2)

Size:96WELLS

Mouse Psen1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	90-101%	84-104%	87-103%	96-105%
  EDTA Plasma (n=5)	87-102%	98-105%	91-105%	87-95%
  Heparin Plasma (n=5)	99-105%	89-103%	85-101%	83-96%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Presenilin 1 (PSEN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Presenilin 1 (PSEN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Presenilin 1 (PSEN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Presenilin 1 (PSEN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	89-104	98
  EDTA Plasma (n=5)	80-95	87
  Heparin Plasma (n=5)	88-95	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Presenilin 1 (PSEN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Presenilin 1 (PSEN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Psen1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Psen1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Psen1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.071 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Presenilin 1 (PSEN1). No significant cross-reactivity or interference between Presenilin 1 (PSEN1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Psen1
• Gene Full Name: presenilin 1
• Alias Symbols: Ad3;Ad3h;P;presenilin-1;Protein S182;PS;PS1;PS-1;S18;S182.
• NCBI Gene Id: 19164
• Protein Name: Presenilin-1
• Description of Target: Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the presence of the other members of the gamma-secretase complex for protease activity (By similarity). Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (PubMed:10421573, PubMed:11517342). Stimulates cell-cell adhesion via its interaction with CDH1; this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin) (PubMed:11226248). Under conditions of apoptosis or calcium influx, cleaves CDH1 (PubMed:11953314). This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling (PubMed:11226248). Required for normal embryonic brain and skeleton development, and for normal angiogenesis (PubMed:9160754, PubMed:10421573, PubMed:12834865).
• Uniprot ID: P49769
• Protein Accession #: NP_032969.1
• Nucleotide Accession #: NM_008943.2
• Protein Size (# AA): 467