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PRL ELISA Kit (Rat) (OKEH02956)

96 Wells
$359.00
In Stock
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Gene Symbol:
PRL
Official Gene Full Name:
prolactin
NCBI Gene Id:
24683
Alias Symbols:
PRL, Prl1a1, PRLB, PRLSD1, Prol, Prolactin, RATPRLSD1, RNPROL
Description of Target:
Prolactin acts primarily on the mammary gland by promoting lactation.
Protein Name:
Prolactin
Swissprot Id:
P01237
Protein Accession #:
NP_036761.1
Nucleotide Accession #:
NM_012629.1
Predicted Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
1.69 ng/mL
Kit Range:
3.12 - 200ng/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology PRL ELISA Kit (Rat) (OKEH02956) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prl has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Prl is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prl captured in well.
Kit Component:
ComponentAmount
Anti-Prl Microplate96 Wells (12 x 8 Well strips)
Prl Lyophilized Standard2
100X Biotinylated Prl Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Prl.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Prl.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
Natural and recombinant Rat Prolactin
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
26 kDa
Protein Size (# AA):
226

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