Prolactin ELISA Kit (Mouse) (OKBB00635)

Catalog No: OKBB00635

Size:96 Wells

Mouse Prolactin ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse Prolactin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Prolactin has been precoated onto 96-well plates. Standards(E.coli, L32-C228) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Prolactin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Prolactin amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	1490	2929	4543	1272	2683	4726
  SD	67.05	158.2	177.2	99.22	153	246
  CV%	4.5	5.4	3.9	7.8	5.7	5.2

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse Prolactin Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse Prolactin Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse Prolactin Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, citrate)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse Prolactin
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: L32-C228
• Description: For quantitative detection of mouse Prolactin in cell culture supernatants, serum and plasma(heparin, citrate).

Target Info

• Gene Symbol: PRL
• Gene Full Name: prolactin
• Alias Symbols: AV290867, Lactogenic Hormone, Luteotropic Hormone, Mammotropin, OTTMUSP00000000651, PRL, Prl1a1, Prol, Prolactin precursor, RATPRLSD1, RNPROL, RP23-351I6.2, RP3-404K8.1
• NCBI Gene Id: 19109
• Protein Name: Prolactin
• Description of Target: Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.
• Uniprot ID: P06879
• Protein Accession #: NP_035294
• Nucleotide Accession #: NM_001163530