PRF1 ELISA Kit (Mouse) (OKCD04461)

Catalog No: OKCD04461

Size:96WELLS

Mouse PRF1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	85-99%	98-105%	87-94%	81-92%
  EDTA Plasma (n=5)	81-98%	86-101%	94-105%	97-105%
  Heparin Plasma (n=5)	78-93%	83-103%	78-95%	80-89%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Perforin 1 (PRF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Perforin 1 (PRF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Perforin 1 (PRF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Perforin 1 (PRF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Perforin 1 (PRF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Perforin 1 (PRF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CX40 Microplate: 96 Wells (12 x 8 Well strips)
  • CX40 Lyophilized Standard: 2
  • 100X CX40 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.062 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Prf1
• Gene Full Name: perforin 1 (pore forming protein)
• Alias Symbols: cytolysin;lymphocyte pore-forming protein;P;P1;pe;perforin-1;Pfn;Pfp;pore forming protein;Prf;Prf-1.
• NCBI Gene Id: 18646
• Protein Name: Perforin-1
• Description of Target: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
• Uniprot ID: P10820
• Protein Accession #: NP_035203.3
• Nucleotide Accession #: NM_011073.3