PRF1 Antibody - FITC Conjugated (OAED00296)

Catalog No: OAED00296

Size:100tests

Flow cytometry analysis of 106 Jurkat cells using Perforin (mouse) Monoclonal Antibody (CB5.4), DyLight488 Conjugate at a concentration of 50 ug/mL

Schematic structure of mouse perforin (recombinant, aa. 98-534). The antibody recognizes an epitope within aa. 402-534.

Product Info

• Predicted Species Reactivity: Mouse
• Clonality: Monoclonal
• Clone: CB5.4
• Isotype: IgG2a
• Host: Rat
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: ICC
• Reconstitution and Storage: Long Term Storage : +4C
  Short Term Storage : +4C
  Handling : Do not freeze/thaw.
  Stable for at least 6 months after receipt when stored at +4C undiluted.
• Immunogen: Recombinant mouse perforin (aa 98-534).
• Concentration: 1mg/ml
• Specificity: Recognizes mouse perforin (region aa 402-534).
• Formulation: Liquid. In PBS containing 0.02% sodium azide.
• Purity: ≥95% (SDS-PAGE).
• Application Info: Immunocytochemistry
• Reference: Fas-Fas Ligand Interactions Are Essential for the Binding to and Killing of Activated Macrophages by {gamma}{delta} T Cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004), Abstract;
  Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem. 277, 15904 (2002), Abstract; Full Text
  Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin: C. Muller & J. Tschopp; J. Immunol. 153, 2470 (1994), Abstract;
  The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes: M. Dupuis, et al.; J. Exp. Med. 177, 1 (1993), Abstract;
  Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004), Abstract;
  Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem. 277, 15904 (2002), Abstract;

Target Info

• Gene Symbol: PRF1
• Gene Full Name: Perforin-1
• Alias Symbols: P1, PFP, HPLH2
• NCBI Gene Id: 5551
• Protein Name: Perforin-1
• Uniprot ID: P14222
• Protein Accession #: NP_001076585.1; NP_005032.2
• Nucleotide Accession #: NM_001083116.1; NM_005041.4