PRDM1 Antibody (OAPB00471)

Catalog No: OAPB00471

Size:100UG

Western blot analysis of Blimp-1 in mouse lung tissue lysate with Blimp-1 antibody at (A) 0.5 and (B) 1ug/mL.

Immunohistochemistry of Blimp-1 in mouse lung tissue with Blimp-1 antibody at 5 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. Blimp-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: Blimp-1 antibody can be stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Blimp-1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Blimp-1. The immunogen is located within amino acids 100 - 150 of Blimp-1.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-PRDM1 (OAPB00471) antibody is Catalog # OEPB02582
• Application Info: Blimp-1 antibody can be used for detection of Blimp-1 by Western blot at 0.5 - 1 ug/ml. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. (Optimal dilution should be determined by user)
• Reference: Angelin-Duclos C, Cattoretti G, Lin K-I, et al. Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression. J. Immunol. 2000; 165:5462-71.
  Reimold AM, Iwakoshi NN, Manis J, et al. Plasma cell differentiation requires the transcription factor XBP-1. Nature 2001; 412:300-7.
  Martins GA, Cimmino L, Shapiro-Shelef M, et al. Transcriptional repressor Blimp-1 regulates T cell homeostasis and function. Nature Immunol. 2006; 7:457-65.;

Target Info

• Gene Symbol: PRDM1
• Gene Full Name: PR/SET domain 1
• Alias Symbols: Beta-interferon gene positive regulatory domain I-binding factor;beta-interferon gene positive-regulatory domain I binding factor;BLIMP1;BLIMP-1;B-lymphocyte-induced maturation protein 1;Positive regulatory domain I-binding factor 1;PR domain 1;PR domain containing 1, with ZNF domain;PR domain zinc finger protein 1;PR domain-containing protein 1;PRDI-BF1;PRDI-binding factor-1.
• NCBI Gene Id: 639
• Protein Name: PR domain zinc finger protein 1|PR domain zinc finger protein 1 isoform 1 [Homo sapiens]
• Description of Target: Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs. Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (By similarity). Binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions (By similarity).
• Uniprot ID: O75626
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001189
• Nucleotide Accession #: NM_001198.3; NM_182907.2