PF4 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03877)

Catalog No: OKCD03877

Size:96WELLS

Mouse PF4 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	83-102%	95-103%	83-97%	80-103%
  EDTA Plasma (n=5)	85-97%	87-103%	86-101%	84-103%
  Heparin Plasma (n=5)	80-96%	90-99%	80-101%	90-103%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Platelet Factor 4 (PF4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Platelet Factor 4 (PF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Platelet Factor 4 (PF4) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90-99	95
  EDTA Plasma (n=5)	98-105	102
  Heparin Plasma (n=5)	85-102	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Factor 4 (PF4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Factor 4 (PF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Pf4 Microplate: 96 Wells (12 x 8 Well strips)
  • Pf4 Lyophilized Standard: 2
  • 100X Pf4 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, platelet-poor plasma and cell culture supernates
• Sensitivity: 2.74 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Pf4
• Gene Full Name: platelet factor 4
• Alias Symbols: chemokine (C-X-C motif) ligand 4;C-X-C motif chemokine 4;Cxcl;Cxcl4;Pf;PF-4;platelet factor 4;Scyb;Scyb4.
• NCBI Gene Id: 56744
• Protein Name: Platelet factor 4
• Description of Target: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation (By similarity).
• Uniprot ID: Q9Z126
• Protein Accession #: NP_064316
• Nucleotide Accession #: NM_019932