CXCL4 Polyclonal Antibody (OABB00400)

Catalog No: OABB00400 (Formerly GWB-BBP323)

Size:100UG

Lane 1: Recombinant Human CXCL4 Protein 10ng
Lane 2: Recombinant Human CXCL4 Protein 5ng
Lane 3: Recombinant Human CXCL4 Protein 2.5ng

IHC(P): Human Lung Cancer Tissue

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Horse|Human
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: CXCL4, Chemokine(C-X-C motif) ligand 4, is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. CXCL4 belongs to the CXC chemokine family. The CXCL4 gene contains 3 exons spanning approximately 1 kb. The gene is encoded on a 10-kb EcoRI fragment of genomic DNA. By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
  Background: CXCL4, Chemokine (C-X-C motif) ligand 4, is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. CXCL4 belongs to the CXC chemokine family. The CXCL4 gene contains 3 exons spanning approximately 1 kb. The gene is encoded on a 10-kb EcoRI fragment of genomic DNA. By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
• Reference: 1. Eisman, R., Surrey, S., Ramachandran, B., Schwartz, E., Poncz, M. Structural and functional
  comparison of the genes for human platelet factor 4 and PF4-alt. Blood 76: 336-344, 1990.
  2. Griffin, C. A., Emanuel, B. S., LaRocco, P., Schwartz, E., Poncz, M. Human platelet factor 4 gene is
  mapped to 4q12-q21. Cytogenet. Cell Genet. 45: 67-69, 1987.
  3. Lambert, M. P., Rauova, L., Bailey, M., Sola-Visner, M. C., Kowalska, M. A., Poncz, M. Platelet
  factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic
  implications. Blood 110: 1153-1160, 2007.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Platelet factor 4(PF4) detection. Tested with WB, IHC-P in Human.

Target Info

• Gene Symbol: PF4
• Gene Full Name: platelet factor 4
• Alias Symbols: chemokine (C-X-C motif) ligand 4;C-X-C motif chemokine 4;CXCL4;iroplact;oncostatin-A;PF-4;platelet factor 4;SCYB4.
• NCBI Gene Id: 5196
• Protein Name: Platelet factor 4
• Description of Target: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
• Uniprot ID: P02776
• Molecular Weight: 10845 MW