PCNA Recombinant Protein (OPCD06015)

Catalog No: OPCD06015

PCNA Recombinant Protein (OPCD06015) using SDS-PAGE

PCNA Recombinant Protein (OPCD06015) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Human|Mouse|Mus musculus|Rat|Rattus norvegicus
• Product Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Host: E.coli
• Conjugation: Unconjugated
• Application: Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Leu6-Ser261
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 200 ug/mL (prior to lyoph)
• Purity: > 95%
• Source: E.coli
• Tag: N-terminal His and GST Tag

Target Info

• Gene Symbol: Pcna
• Gene Full Name: proliferating cell nuclear antigen
• Alias Symbols: cyclin;Pcna/cyclin;PCNAR;proliferating cell nuclear antigen.
• NCBI Gene Id: 18538|25737
• Protein Name: Proliferating cell nuclear antigen
• Description of Target: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (By similarity).
• Uniprot ID: P04961|P17918
• Protein Accession #: NP_002583.1
• Nucleotide Accession #: NM_002592.2
• Protein Size (# AA): Leu6~Ser261
• Molecular Weight: 58kDa