P4HB Recombinant Protein (Human) (OPED00114)

Catalog No: OPED00114

Size:50 ug

SDS-PAGE analysis: Lane 1: MW marker, Lane 2: 0.5ug, Lane 3: 1ug, Lane 4: 2ug, Lane 5: 5ug PDI (human), (recombinant) (His-tag).

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Liquid. In 50mM TRIS, pH 7.5, containing 150mM sodium chloride, 1mM DTT, 1mM EDTA, and 5% glycerol.
• Host: E. Coli
• Application: WB
• Additional Information: The mammalian protein disulfide-isomerase (PDI) family encompasses several highly divergent proteins involved in the processing and maturation of secretory proteins in the ER by catalyzing the rearrangement of disulfide bonds. PDI, an abundant protein of the ER (>400uM), contains a carboxy-terminal retention signal sequence, KDEL, similar to that of BiP and Grp94. The PDI proteins are characterized by the presence of one or more domains of ~95-110 amino acids related to the cytoplasmic protein thioredoxin. All but the PDI-D subfamily are composed entirely of repeats of such domains, with at least one domain containing – and one domain lacking – a redox-active Cys-X-X-Cys tetrapeptide. In addition to roles as redox catalysts and isomerases, PDI proteins perform such functions as peptide binding and cell adhesion, and may conduct chaperone activities1. Platelet surface thiols and disulfides play an important role in platelet responses. Catalytically active PDI resides on platelet surfaces where it mediates platelet aggregation and secretion by reducing disulfide bonds, thus exposing fibrinogen receptors in platelets.
• Reconstitution and Storage: Store at -80C
• Purification: Purified by multi-step chromatography.
• Purity: >95% (SDS-PAGE; Western blot)
• Application Info: Western blot control.

Target Info

• Gene Symbol: P4HB
• Gene Full Name: prolyl 4-hydroxylase subunit beta
• Alias Symbols: cellular thyroid hormone-binding protein;CLCRP1;collagen prolyl 4-hydroxylase beta;DSI;ERBA2L;GIT;glutathione-insulin transhydrogenase;P4Hbeta;p55;PDI;PDIA1;PHDB;PO4DB;PO4HB;procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide;PROHB;Prolyl 4-hydroxylase subunit beta;prolyl 4-hydroxylase, beta polypeptide;protein disulfide isomerase family A, member 1;protein disulfide isomerase/oxidoreductase;protein disulfide isomerase-associated 1;protein disulfide-isomerase;protocollagen hydroxylase;testicular secretory protein Li 32;thyroid hormone-binding protein p55.
• NCBI Gene Id: 5034
• Protein Name: Protein disulfide-isomerase
• Description of Target: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).
• Uniprot ID: P07237
• Protein Accession #: NP_035746.2
• Nucleotide Accession #: NM_011616.2
• Protein Size (# AA): Recombinant
• Molecular Weight: 58 kDa