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- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OCT4 Antibody (OAAD00322) - OAAD00322 |
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Predicted Species Reactivity | Human |
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Clonality | Monoclonal |
Clone | 3A5 |
Isotype | IgG1 |
Host | Mouse |
Application | WB |
Reconstitution and Storage | Store at 4C, for long term storage, store at -20C. |
Immunogen | Purified recombinant fragment of OCT4 expressed in E. Coli. |
Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application Info | Western Bloting: 1/500 - 1/2000. ELISA: Propose dilution 1/10000. Not yet tested in other applications. Determining optimal working dilutions by titration test. |
Reference | 1. Stem Cells. 2009 Mar;27(3):543-9. 2. Genome Biol. 2008;9(12):R172. |
Gene Symbol | POU5F1 |
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Alias Symbols | OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4 |
NCBI Gene Id | 5460 |
Protein Name | POU domain, class 5, transcription factor 1 |
Description of Target | Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation. Oct4 is renowned as a transcription factor expressed by undifferentiated embryonic stem cells and embryonic germ cells. |
Uniprot ID | Q01860 |
Molecular Weight | 39kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "OCT4 Antibody (OAAD00322)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "OCT4 Antibody (OAAD00322)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "OCT4 Antibody (OAAD00322)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "OCT4 Antibody (OAAD00322)"?
This target may also be called "OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4" in publications.
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What is the shipping cost for "OCT4 Antibody (OAAD00322)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "OCT4 Antibody (OAAD00322)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "OCT4 Antibody (OAAD00322)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "39kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "OCT4 Antibody (OAAD00322)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "POU5F1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "POU5F1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "POU5F1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "POU5F1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "POU5F1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "POU5F1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.