Catalog No: OKBB00232
Size:96 Tests
Price: $526.00
SKU
OKBB00232
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Human | |||||||||||||||||||||||||||||||||||
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Application | ELISA | |||||||||||||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | |||||||||||||||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | |||||||||||||||||||||||||||||||||||
ELISA Kit Principle | Aviva's human Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards(NS0, Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Neurotrophin-3 amount of sample captured in plate. | |||||||||||||||||||||||||||||||||||
ELISA Kit Range | 15.6 pg/ml - 1,000 pg/ml | |||||||||||||||||||||||||||||||||||
ELISA Kit Reproducibility |
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ELISA Kit Component |
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Additional Information | Range: 15.6pg/ml-1000pg/ml | |||||||||||||||||||||||||||||||||||
:: | Principle: Aviva’s human Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards (sf21, Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Neurotrophin-3 amount of sample captured in plate. | |||||||||||||||||||||||||||||||||||
:: | Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended. 2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case. 3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes. 4. Duplicate well assay is recommended for both standard and sample testing. 5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate. 6. Don’t reuse tips and tubes to avoid cross contamination. 7. To avoid to use the reagents from different batches together. 8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before using. Background: Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.1 NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis.2 NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation.3 NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo.4 NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.1 | |||||||||||||||||||||||||||||||||||
Reconstitution and Storage | Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) | |||||||||||||||||||||||||||||||||||
Sample Type | cell culture supernates and serum | |||||||||||||||||||||||||||||||||||
Sensitivity | <2 pg/ml | |||||||||||||||||||||||||||||||||||
Reference | 1. Ozcelik, T.; Rosenthal, A.; Francke, U. Chromosomal mapping of brain-derived neurotrophic factor and neurotrophin-3 genes in man and mouse. Genomics 10: 569-575, 1991. 2. Tessarollo, L.; Vogel, K. S.; Palko, M. E.; Reid, S. W.; Parada, L. F. Targeted mutation in the neurotrophin-3 gene results in loss of muscle sensory neurons. Proc. Nat. Acad. Sci. 91: 11844-11848, 1994. 3. Donovan, M. J.; Hahn, R.; Tessarollo, L.; Hempstead, B. L. Identification of an essential nonneuronal function of neurotrophin 3 in mammalian cardiac development. Nature Genet. 14: 210-213, 1996. 4. Kalcheim, C.; Carmeli, C.; Rosenthal, A. Neurotrophin 3 is a mitogen for cultured neural crest cells. Proc. Nat. Acad. Sci. 89: 1661-1665, 1992. | |||||||||||||||||||||||||||||||||||
Specificity | Natural and recombinant human Neurotrophin-3 | |||||||||||||||||||||||||||||||||||
Immunogen | Expression system for standard: NS0; Immunogen sequence: Y139-T257 | |||||||||||||||||||||||||||||||||||
Description | For quantitative detection of human Neurotrophin-3 in cell culture supernatants and serum. |
Gene Symbol | NTF3 |
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Gene Full Name | neurotrophin 3 |
Alias Symbols | AI316846, AI835689, HDNF, MGC129711, Nerve growth factor 2, Neurotrophic factor, Neurotrophin 3 (HDNF/NT 3), Neurotrophin 3 precursor, Neurotrophin3, NGF 2, NGF2, NT3, NTF 3, NTF3 |
NCBI Gene Id | 4908 |
Protein Name | Neurotrophin-3 |
Description of Target | Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo.4 NT-3 has been mapped to human chromosome 12p and mouse chromosome 6. |
Uniprot ID | P20783 |
Protein Accession # | NP_001096124.1 |
Nucleotide Accession # | NM_001102654.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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