NT-3 ELISA Kit (Rat) (OKBB00234)

Catalog No: OKBB00234

Size:96 Tests

Rat NTF-3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's rat Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards(NS0, Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat Neurotrophin-3 amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	94	382	624	123	417	716
  St. Dev.	4.42	20.63	37.44	6.4	24.2	50.84
  %CV	4.70	5.40	6	5.20	5.80	7.10

• ELISA Kit Component:
  • Lyophilized recombinant human MMP-7 standard: 10 ng/tube x 2
  • Anti-human MMP-7 Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human MMP-7 antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 15.6pg/ml-1000pg/ml
  Principle: Aviva’s rat Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat Neurotrophin-3 specific-specific polyclonal antibodies were precoated onto 96-well plates. The rat specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Neurotrophin-3 amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system.1 NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis.2 NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation.3 NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo.4 NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.1
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates and serum
• Sensitivity: <2 pg/ml
• Predicted Homology Based on Immunogen Sequence: Cross-reactivates with NT-4 <2.5%, and no detectable cross-reactivity with any other cytokine
• Reference: 1. Ozcelik, T.; Rosenthal, A.; Francke, U. Chromosomal mapping of brain-derived neurotrophic factor and
  neurotrophin-3 genes in man and mouse. Genomics 10: 569-575, 1991.
  2. Tessarollo, L.; Vogel, K. S.; Palko, M. E.; Reid, S. W.; Parada, L. F. Targeted mutation in the neurotrophin-3 gene
  results in loss of muscle sensory neurons. Proc. Nat. Acad. Sci. 91: 11844-11848, 1994.
  3. Donovan, M. J.; Hahn, R.; Tessarollo, L.; Hempstead, B. L. Identification of an essential nonneuronal function of
  neurotrophin 3 in mammalian cardiac development. Nature Genet. 14: 210-213, 1996.
  4. Kalcheim, C.; Carmeli, C.; Rosenthal, A. Neurotrophin 3 is a mitogen for cultured neural crest cells. Proc. Nat. Acad.
  Sci. 89: 1661-1665, 1992.
• Specificity: Natural and recombinant rat Neurotrophin-3
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Y139-T257
• Description: For quantitative detection of rat Neurotrophin-3 in cell culture supernatants and serum.

Target Info

• Gene Symbol: NTF3
• Gene Full Name: neurotrophin 3
• Alias Symbols: AI316846, AI835689, HDNF, MGC129711, Nerve growth factor 2, Neurotrophic factor, Neurotrophin 3 (HDNF/NT 3), Neurotrophin 3 precursor, Neurotrophin3, NGF 2, NGF2, NT3, NTF 3, NTF3
• NCBI Gene Id: 81737
• Protein Name: Neurotrophin-3
• Description of Target: Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo.4 NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.
• Uniprot ID: P18280
• Protein Accession #: NP_001257797.1
• Nucleotide Accession #: NM_001270868.1