NPPB Chemi-Luminescent ELISA Kit (Dog) (OKCD05539)

Catalog No: OKCD05539

Size:96 Wells

Product Info

• Predicted Species Reactivity: Dog
• Application: ELISA
• ELISA Kit Detection Method: Chemiluminescent, visible wavelength light
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology NPPB Chemi-Luminescent ELISA Kit (Dog) (OKCD05539) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NPPB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPPB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample NPPB.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level NPPB. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level NPPB using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • NPPB Microplate: 96 Wells (12 x 8 Well strips)
  • NPPB Lyophilized Standard: 2
  • 100X NPPB HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 9 mL
  • Substrate Diluent: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.55pg/mL

Target Info

• Gene Symbol: NPPB
• Gene Full Name: natriuretic peptide B
• Alias Symbols: BNP, Iso-ANP
• NCBI Gene Id: 487441
• Protein Name: Natriuretic peptides B
• Description of Target: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
• Uniprot ID: P16859