NPPB ELISA Kit (Rat) (OKEH03140)

Catalog No: OKEH03140

Size:96T

Rat NPPB ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Nppb ELISA Kit (Rat) (OKEH03140) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nppb has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Nppb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nppb captured in the well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 104%
• ELISA Kit Reproducibility: Mean Intra-Assay Precision - ≤ 5.6%, n = 20
  Mean Inter-Assay Precision - ≤ 8.1%, n = 20
• ELISA Kit Component:
  • Anti-Nppb Microplate: 96 Wells (12 x 8 Well strips)
  • Nppb Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Nppb Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 15.69 pg/mL
• Specificity: Natural and recombinant Rat Natriuretic peptides B
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Nppb
• Gene Full Name: natriuretic peptide B
• Alias Symbols: Bnf;BNP;Brain natriuretic factor;brain natriuretic factor prohormone;brain natriuretic peptide;gamma-brain natriuretic peptide;iso-ANP;natriuretic peptide precursor B;natriuretic peptide precursor type B;natriuretic peptides B;preproBNP;proBNP.
• NCBI Gene Id: 25105
• Protein Name: P13205
• Description of Target: Natriuretic peptides B
• Uniprot ID: https://www.uniprot.org/uniprot/P13205
• Protein Accession #: NP_113733.1
• Nucleotide Accession #: NM_031545.1
• Protein Size (# AA): 121
• Molecular Weight: 14 kDa