NODAL ELISA Kit (Mouse) (OKCA02180)

Catalog No: OKCA02180

Size:96 Wells

Mouse NODAL ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	87	82-91
  Serum (n=4)	1:2	102	98-107
  Serum (n=4)	1:4	98	94-102
  Serum (n=4)	1:8	87	82-93

• ELISA Kit Principle: Aviva Systems Biology Nodal ELISA Kit (Mouse) (OKCA02180) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NODAL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NODAL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NODAL captured in well.
• ELISA Kit Range: 15.6 pg/mL-1,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	92	88-96
  EDTA plasma (n=4)	84	80-88

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-NODAL Microplate: 96 Wells (12 x 8 Well strips)
  • NODAL Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated NODAL Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 3.9 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NODAL
• Alias Symbols: Tg.413d
• NCBI Gene Id: 18119
• Protein Name: Nodal
• Description of Target: Essential for mesoderm formation and axial patterning during embryonic development.
• Uniprot ID: P43021
• Protein Accession #: NP_038639
• Nucleotide Accession #: NM_013611