Catalog No: OKEH02224
Size:96T
Price: $600.00
SKU
OKEH02224
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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NODAL ELISA Kit (Human) : 96 Wells (OKEH02224)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology NODAL ELISA Kit (Human) (OKEH02224) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NODAL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NODAL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NODAL captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 111%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.4% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
NODAL Microplate96 Wells (12 x 8 Well strips)
NODAL Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated DIO3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity39 pg/mL
SpecificityNatural and recombinant Human Nodal homolog
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNODAL
Gene Full Namenodal growth differentiation factor
Alias SymbolsHTX5;nodal homolog.
NCBI Gene Id4838
Protein NameQ96S42
Description of TargetNodal homolog
Uniprot IDhttps://www.uniprot.org/uniprot/Q96S42
Protein Accession #NP_060525.3
Nucleotide Accession #NM_018055.4
Protein Size (# AA)347
Molecular Weight40 kDa
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