Catalog No: OKEH05486
Size:96T
Price: $625.00
SKU
OKEH05486
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
NLRP3 ELISA Kit (Mouse) (OKEH05486)
Predicted Species Reactivity | Mouse, Mus musculus | ||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Nlrp3 ELISA Kit (Mouse) (OKEH05486) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nlrp3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nlrp3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nlrp3 captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 94% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision. Mean Intra-Assay Precision - <4.9%, n = 20 Mean Inter-Assay Precision - <7.1%, n = 20 | ||||||||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.058 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Mouse NACHT, LRR and PYD domains-containing protein 3 | ||||||||||||||||||||||
Publications | Evidences of CTLA-4 and PD-1 Blocking Agents-Induced Cardiotoxicity in Cellular and Preclinical Models. J Pers Med. 10, NULL (2020). 33086484 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Description |
Gene Symbol | Nlrp3 |
---|---|
Gene Full Name | NLR family, pyrin domain containing 3 |
Alias Symbols | AGTAVPRL;AII/AVP;Ci;Cias1;cold autoinflammatory syndrome 1 protein homolog;cry;cryopyrin;FCAS;FCU;mast cell maturation-associated-inducible protein 1;Mmig;Mmig1;MWS;N;NACHT, LRR and PYD domains-containing protein 3;NACHT/LRR/pyrin domain-containing protein 3;NALP3;Pyp;Pypaf1;PYRIN-containing APAF1-like protein 1. |
NCBI Gene Id | 216799 |
Protein Name | Q8R4B8 |
Description of Target | NACHT, LRR and PYD domains-containing protein 3 |
Uniprot ID | https://www.uniprot.org/uniprot/Q8R4B8 |
Protein Accession # | NP_665826.1 |
Nucleotide Accession # | NM_145827.3 |
Protein Size (# AA) | 1033 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review
We found other products you might like!