NLRP3 Antibody (OABF00351)

Catalog No: OABF00351

Size:100UL

HeLa lysates probed with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated (OABF00351) at 1:300 overnight at 4C. Followed by conjugation to secondary antibody at 1:5000 for 90 min at 37C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti Cryopyrin/CIAS1/NALP3 Polyclonal Antibody, Unconjugated (OABF00351) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated (OABF00351) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-Cryopyrin Polyclonal Antibody, Unconjugated (OABF00351) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Rat
• Product Format: Liquid Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Subcellular location: Cytoplasm, Nucleus, Secreted
• Reconstitution and Storage: -20°C
• Immunogen: KLH conjugated synthetic peptide derived from human NLRP3
• Purification: Protein A Purified
• Concentration: 1 ug/ul
• Application Info: WB: 1:300~1000
  ELISA: 1:500~1000
  FC: 1:20~100
  ICC: 1:100~500
  IHC-P: 1:200~400
  IHC-F: 1:100~500
  Fluorescent (IHC-P): 1:50~200
  Fluorescent (IHC-F): 1:50~200
  Fluorescent-ICC: 1:50~200

Target Info

• Gene Symbol: NLRP3
• Gene Full Name: NLR family pyrin domain containing 3
• Alias Symbols: AGTAVPRL;AII;Angiotensin/vasopressin receptor AII/AVP-like;AVP;C1orf7;caterpiller protein 1.1;CIAS1;CLR1.1;cold autoinflammatory syndrome 1 protein;cold-induced autoinflammatory syndrome 1 protein;cryopyrin;cryopyrin, NACHT, LRR and PYD domains - containing protein 3;deafness, autosomal dominant 34;DFNA34;FCAS;FCAS1;FCU;KEFH;MWS;NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3;NACHT, LRR and PYD containing protein 3;NACHT, LRR and PYD domains-containing protein 3;NALP3;nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3;PYPAF1;PYRIN-containing APAF1-like protein 1.
• NCBI Gene Id: 114548
• Protein Name: NACHT, LRR and PYD domains-containing protein 3
• Description of Target: As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (and possibly CASP4 and CASP5). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP3 inflammasome is also required for HMGB1 secretion (PubMed:22801494). The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33 (PubMed:23871209). Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth (By similarity). During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (By similarity).
• Uniprot ID: Q96P20