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Datasheets/Manuals | Printable datasheet for OAAB17106 |
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Predicted Species Reactivity | Human |
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Product Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB, IHC |
Additional Information | Precautions: NLK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
:: | Function: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK- SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1 Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1 |
:: | Cellular Location: Nucleus (By similarity). Cytoplasm (By similarity). Note=Predominantly nuclear. A smaller fraction is cytoplasmic (By similarity) |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This NLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-436 amino acids from the C-terminal region of human NLK. |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Application Info | WB: 1:1000 IHC-P: 1:50~100 |
Gene Symbol | NLK |
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Alias Symbols | NLK, LAK1, Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1 |
NCBI Gene Id | 51701 |
Protein Name | Serine/threonine-protein kinase NLK |
Description of Target | Purified Rabbit Polyclonal Antibody (Pab) |
Uniprot ID | Q9UBE8 |
Protein Accession # | NP_057315.3 |
Molecular Weight | 58283 Da |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "NLK Antibody - C-terminal region (OAAB17106)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "NLK Antibody - C-terminal region (OAAB17106)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "NLK Antibody - C-terminal region (OAAB17106)" provided in?
This item is provided in "Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "NLK Antibody - C-terminal region (OAAB17106)"?
This target may also be called "NLK, LAK1, Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1" in publications.
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What is the shipping cost for "NLK Antibody - C-terminal region (OAAB17106)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "NLK Antibody - C-terminal region (OAAB17106)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "NLK Antibody - C-terminal region (OAAB17106)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "58283 Da".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "NLK Antibody - C-terminal region (OAAB17106)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "NLK"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "NLK"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "NLK"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "NLK"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "NLK"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "NLK"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.