NANOG Recombinant Protein (OPCD05573)

Catalog No: OPCD05573

NANOG Recombinant Protein (OPCD05573) using SDS-PAGE

NANOG Recombinant Protein (OPCD05573) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Host: E.coli
• Conjugation: Unconjugated
• Application: Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Met1-Val305
  Subcellular Location: Nucleus
  Isoelectric Point: 6.3
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 800 ug/mL (prior to lyoph)
• Purity: > 97%
• Source: E.coli
• Tag: N-terminal His and GST Tag

Target Info

• Gene Symbol: NANOG
• Gene Full Name: Nanog homeobox
• Alias Symbols: homeobox protein NANOG;homeobox transcription factor Nanog;homeobox transcription factor Nanog-delta 48.
• NCBI Gene Id: 79923
• Protein Name: Homeobox protein NANOG
• Description of Target: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
• Uniprot ID: Q9H9S0
• Protein Accession #: NP_001284627.1
• Nucleotide Accession #: NM_001297698.1
• Protein Size (# AA): Met1~Val305
• Molecular Weight: 65kDa