Myogenin Antibody (OAAF05854)

Catalog No: OAAF05854

Size:100 ug

Western blot analyzes of Myogenin antibody in extracts from A549 cells.

Product Info

• Predicted Species Reactivity: Human|Mouse|Porcine|Rat
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Myogenin.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Concentration: 1 mg/ml
• Specificity: Myogenin antibody detects endogenous levels of Myogenin.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:500 - 1:1000
  ELISA: 1:10000

Target Info

• Gene Symbol: MYOG
• Gene Full Name: myogenin
• Alias Symbols: bHLHc3;class C basic helix-loop-helix protein 3;myf-4;MYF4;myogenic factor 4;myogenin.
• NCBI Gene Id: 4656
• Protein Name: Myogenin
• Description of Target: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).
• Uniprot ID: P15173
• Molecular Weight: 25 kDa