MYOG Antibody - N-terminal region (OAAB06865)

Catalog No: OAAB06865

Size:400ul

MYOG Antibody (N-term) (Cat. #OAAB06865) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MYOG antibody detected the MYOG protein (arrow).

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Monoclonal
• Clone: 304CT11.6.1
• Isotype: IgG1,k
• Host: Mouse
• Application: WB
• Additional Information: Function: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin- remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts (By similarity).
  Cellular Location: Nucleus. Note=Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin- remodeling and transcription initiation in developing embryos
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MYOG antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of human MYOG.
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:100
• Intended Use: MYOG Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Target Info

• Gene Symbol: MYOG
• Alias Symbols: MYF4, myf-4, bHLHc3
• NCBI Gene Id: 4656
• Protein Name: Myogenin
• Description of Target: Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle.
• Uniprot ID: P15173
• Protein Accession #: NP_002470.2
• Molecular Weight: 25037 Da