MYC ELISA Kit (Human) (OKCD07288)

Catalog No: OKCD07288

Size:96WELLS

MYC ELISA Kit (Human) (OKCD07288) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • MYC Microplate: 96 Wells (12 x 8 Well strips)
  • MYC Lyophilized Standard: 2
  • 100X MYC HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.59ng/mL

Target Info

• Gene Symbol: MYC
• Gene Full Name: MYC proto-oncogene, bHLH transcription factor
• Alias Symbols: avian myelocytomatosis viral oncogene homolog;bHLHe39;class E basic helix-loop-helix protein 39;c-Myc;MRTL;myc proto-oncogene protein;MYCC;myc-related translation/localization regulatory factor;proto-oncogene c-Myc;transcription factor p64;v-myc avian myelocytomatosis viral oncogene homolog;v-myc myelocytomatosis viral oncogene homolog.
• NCBI Gene Id: 4609
• Protein Name: Myc proto-oncogene protein
• Description of Target: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000).
• Uniprot ID: P01106-2
• Protein Accession #: NP_002458.2
• Nucleotide Accession #: NM_002467.4