MSLN Antibody (OARA02143)

Catalog No: OARA02143

Size:25UL

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid (sterile filtered)
• Clonality: Monoclonal
• Clone: MN-1
• Host: Mouse
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Label: Unconjugated
  
• Reconstitution and Storage: -20°C|See manual
• Immunogen: This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
• Purification: This product was purified from tissue culture supernatant fluid by Protein A chromatography.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Specificity: This antibody is directed against human mesothelin protein. Cross reactivity with homologues from other sources has not been tested.
• Application Info: ELISA: 1:10,000 - 1:50,000
  WB: 1:1,000
  IHC: 1:100
• Application Data: This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.
• Reference: Chang K, Pastan I, Willingham MC. (1992) Isolation and characterization of a monoclonal antibody reactive with ovarian cancers and normal mesothelium. Int J Cancer 50:373-81.Argani P, Iacobuzio-Danahue C, Ryu B, et al. (2001) Mesothelin is overexpressed in the vast majority of ductal adenocarcinomas of the pancreas: identification of a new pancreatic cancer marker by serial analysis of gene expression (SAGE). Clin Cancer Res 7:3862-8.Chang K, Pastan I.(1996) Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesothelioma, and ovarian cancers. Proc Natl Acad Sci U S A 93:136-40.

Target Info

• Gene Symbol: MSLN
• Gene Full Name: mesothelin
• Alias Symbols: CAK1 antigen;megakaryocyte potentiating factor;mesothelin;MPF;pre-pro-megakaryocyte-potentiating factor;SMRP;soluble MPF mesothelin related protein.
• NCBI Gene Id: 10232
• Protein Name: Mesothelin
• Description of Target: Membrane-anchored forms may play a role in cellular adhesion.
• Uniprot ID: Q13421
• Protein Accession #: NP_001170826.1; NP_005814.2; NP_037536.2; XP_005255091.1; XP_006720900.1
• Nucleotide Accession #: NM_001177355.1; NM_005823.5; NM_013404.4; XM_005255034.3; XM_006720837.2