MID1 Antibody (OAAJ01919)

Catalog No: OAAJ01919

Size:100 ul

Western blot analysis on A549 and HuvEc cell lysate using TRI18 Antibody

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC, IF, ICC
  Function: Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.
  Subcellular Location: Cytoplasm, Cytoskeleton, Spindle, Note:Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.
  Tissue Specificity: In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.
• Reconstitution and Storage: Store at -20C. Stable for 12 months from date of receipt.
• Immunogen: A synthesized peptide derived from human TRI18
• Purification: Affinity-chromatography
• Peptide Sequence: METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVSYCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQIIGTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phosphorylated on serine and threonine residues.
• Specificity: TRI18 antibody detects endogenous levels of total TRI18
• Application Info: WB: 1:500~3000
  IHC: 1:50~200
  IF/ICC: 1:100~500

Target Info

• Gene Symbol: MID1
• Gene Full Name: midline 1
• Alias Symbols: OS, FXY, OSX, OGS1, XPRF, BBBG1, GBBB1, MIDIN, RNF59, ZNFXY, TRIM18
• NCBI Gene Id: 4281
• Description of Target: MID1 may have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation. Belongs to the TRIM/RBCC family. A retroviral element acts as an alternative tissue- specific promoter for this gene. The LTR of an HERV-E element enhances the expression in placenta and embryonic kidney. Homodimer or heterodimer with MID2. Interacts with IGBP1. Two isoforms of the human protein are produced by alternative splicing.
• Uniprot ID: O15344
• Molecular Weight: 75 kDa