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Datasheets/Manuals | Printable datasheet for MED21 Antibody (OAAF02413) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human MED21. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | MED21 antibody detects endogenous levels of total MED21 protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | IHC: 1:50~1:100; ELISA: 1:5000 |
Reference | Chao D.M., Nature 380:82-85(1996). Maldonado E., Nature 381:86-89(1996). Oelgeschlaeger T., Mol. Cell 1:925-931(1998). |
Gene Symbol | MED21 |
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Gene Full Name | mediator complex subunit 21 |
Alias Symbols | hSrb7;Mediator complex subunit 21;mediator of RNA polymerase II transcription subunit 21;RNA polymerase II holoenzyme component SRB7;RNAPII complex component SRB7;SRB7;SRB7 suppressor of RNA polymerase B homolog;SURB7. |
NCBI Gene Id | 9412 |
Protein Name | Mediator of RNA polymerase II transcription subunit 21 |
Description of Target | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. |
Uniprot ID | Q13503 |
Protein Accession # | NP_004255.2 |
Molecular Weight | 15 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "MED21 Antibody (OAAF02413)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "MED21 Antibody (OAAF02413)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "MED21 Antibody (OAAF02413)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "MED21 Antibody (OAAF02413)"?
This target may also be called "hSrb7;Mediator complex subunit 21;mediator of RNA polymerase II transcription subunit 21;RNA polymerase II holoenzyme component SRB7;RNAPII complex component SRB7;SRB7;SRB7 suppressor of RNA polymerase B homolog;SURB7." in publications.
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What is the shipping cost for "MED21 Antibody (OAAF02413)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "MED21 Antibody (OAAF02413)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "MED21 Antibody (OAAF02413)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "15 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "MED21 Antibody (OAAF02413)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MED21"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MED21"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MED21"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MED21"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MED21"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MED21"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.