MED17 Antibody (OAGA04959)

Catalog No: OAGA04959

Size:100 ul

Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using MED17 antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using MED17 antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human TRAP80. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
• Concentration: 1 mg/ml
• Application Info: ICC/IF: 1:100~1,000
  IHC-P: 1:100~1,000
  WB: 1:500~3,000
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: MED17
• Gene Full Name: mediator complex subunit 17
• Alias Symbols: SRB4, CRSP6, CRSP77, DRIP80, TRAP80
• NCBI Gene Id: 9440
• Protein Name: mediator of RNA polymerase II transcription subunit 17
• Description of Target: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
• Uniprot ID: Q9NVC6
• Protein Accession #: NP_004259.3
• Nucleotide Accession #: NM_004268.4
• Molecular Weight: 73 kDa