MBD3 Antibody - N-terminal region (ARP40170_P050)

Catalog No: ARP40170_P050

WB Suggested Anti-MBD3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Transfected 293T

Host: Mouse
Target Name: MBD3
Sample Tissue: Mouse Brain
Antibody Dilution: 1ug/ml

Product Info

• Tested Species Reactivity: Mouse
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MBD3
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 85%; Dog: 100%; Guinea Pig: 100%; Horse: 79%; Human: 100%; Mouse: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: SKMNKSRQRVRYDSSNQVKGKPDLNTALPVRQTASIFKQPVTKITNHPSN
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-MBD3 (ARP40170_P050) antibody is Catalog # AAP40170 (Previous Catalog # AAPP20913)
• Reference: Brown,S.E. (2007) Mol. Cell. Biol. 27 (13), 4938-4952

Target Info

• Gene Symbol: MBD3
• Gene Full Name: Methyl-CpG binding domain protein 3
• Alias Symbols: -
• NCBI Gene Id: 53615
• Protein Name: Methyl-CpG-binding domain protein 3
• Description of Target: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: O95983
• Protein Accession #: NP_003917
• Nucleotide Accession #: NM_003926
• Protein Size (# AA): 291
• Molecular Weight: 33kDa
• Protein Interactions: SDCBP; KRT15; ICA1; GOLGA2; CEP76; GATAD2B; RCN3; TRIM54; ZNF277; CEP250; ZBTB16; UBC; RARA; KDM1A; HDAC2; HDAC1; MMS19; HIST3H3; GATAD2A; INO80; MTA2; BAZ1B; SMARCA5; RBBP7; RBBP4; HNRNPM; IGBP1; FUS; EWSR1; DDX3X; DDB1; CHD4; CHD3; TAB2; DNMT1; MTA1; NR