MAP2K7 Antibody (Phospho-Ser271) (OAAF00458)

Catalog No: OAAF00458 (Formerly GWB-ASB901)

Size:100 ug

Western blot analysis of extracts from NIH-3T3 cells treated with insulin 0.01U/ml 15' and NIH-3T3 cells treated with EGF 200ng/ml 30', using MAP2K7 (Phospho-Ser271) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K7 (Phospho-Ser271) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Modification Sites: H: S271, M: S287, R: S271
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MAP2K7 around the phosphorylation site of Ser271.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phospho
• Specificity: MAP2K7 (Phospho-Ser271) Antibody detects endogenous levels of MAP2K7 only when phosphorylated at Ser271.
• Application Info: WB: 1:500~1:1,000
  IHC: 1:50~1:100
  ELISA: 1:20,000
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: MAP2K7
• Gene Full Name: mitogen-activated protein kinase kinase 7
• Alias Symbols: c-Jun N-terminal kinase kinase 2;dual specificity mitogen-activated protein kinase kinase 7;JNK-activating kinase 2;JNKK2;MAP kinase kinase 7;MAPK/ERK kinase 7;MAPKK7;MEK;MEK 7;MKK7;PRKMK7;SAPK kinase 4;SAPKK4;SAPKK-4;stress-activated protein kinase kinase 4.
• NCBI Gene Id: 5609
• Protein Name: Dual specificity mitogen-activated protein kinase kinase 7
• Description of Target: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.
• Uniprot ID: O14733
• Protein Accession #: NP_660186.1
• Molecular Weight: 47 kDa