LGALS3 Antibody (OAAF01805)

Catalog No: OAAF01805 (Formerly GWB-ASD077)

Size:100 ug

Western blot analysis of extracts from HeLa cells, using Galectin 3 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Galectin 3 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of NIH-3T3 cells, using Galectin 3 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Galectin 3.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: Galectin 3 antibody detects endogenous levels of total Galectin 3 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:5000
• Reference: Miliyun G. Chiu, Am. J. Pathol., Dec 2006; 169: 1925 - 1938.
  Luciana Kohatsu, J. Immunol., Oct 2006; 177: 4718 - 4726.
  Brianna N. Stillman, J. Immunol., Jan 2006; 176: 778 - 789.
  Julie Nieminen, J. Leukoc. Biol., Nov 2005; 78: 1127 - 1135.

Target Info

• Gene Symbol: LGALS3
• Gene Full Name: galectin 3
• Alias Symbols: 35 kDa lectin;advanced glycation end-product receptor 3;carbohydrate-binding protein 35;CBP35;epididymis secretory sperm binding protein;GAL3;galactose-specific lectin 3;Galactoside-binding protein;GALBP;galectin-3;GALIG;IgE-binding protein;L31;L-31;laminin-binding protein;lectin L-29;lectin, galactoside-binding, soluble, 3;LGALS2;MAC2;MAC-2 antigen.
• NCBI Gene Id: 3958
• Protein Name: Galectin-3
• Description of Target: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
• Uniprot ID: P17931
• Protein Accession #: NP_002297.2
• Molecular Weight: 26 kDa