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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for Lck Antibody (OAAF00787) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human Lck. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | Lck (Ab-192) antibody detects endogenous levels of total Lck protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; IF: 1:100~1:500; ELISA: 1:1000 |
Reference | K M Tchou-Wong, Mol. Cell. Biol., Jan 1992; 12: 394 - 401. Marit Inngjerdingen, Blood, May 2002; 99: 4318 - 4325. K Lin, Int. Immunol., Feb 1997; 9: 291 - 306. Kavita Tewari, PNAS, Oct 2006; 103: 16388 - 16393. |
Gene Symbol | LCK |
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Gene Full Name | LCK proto-oncogene, Src family tyrosine kinase |
Alias Symbols | IMD22;leukocyte C-terminal Src kinase;LSK;lymphocyte cell-specific protein-tyrosine kinase;p56(LSTRA) protein-tyrosine kinase;p56lck;p56-LCK;pp58lck;Protein YT16;Proto-oncogene Lck;proto-oncogene tyrosine-protein kinase LCK;t cell-specific protein-tyrosine kinase;T-lymphocyte specific protein tyrosine kinase p56lck;tyrosine-protein kinase Lck;YT16. |
NCBI Gene Id | 3932 |
Protein Name | Tyrosine-protein kinase Lck |
Description of Target | Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. Interacts with FYB2 (PubMed:27335501). |
Uniprot ID | P06239 |
Protein Accession # | NP_001036236.1 |
Molecular Weight | 58 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Lck Antibody (OAAF00787)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "Lck Antibody (OAAF00787)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Lck Antibody (OAAF00787)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Lck Antibody (OAAF00787)"?
This target may also be called "IMD22;leukocyte C-terminal Src kinase;LSK;lymphocyte cell-specific protein-tyrosine kinase;p56(LSTRA) protein-tyrosine kinase;p56lck;p56-LCK;pp58lck;Protein YT16;Proto-oncogene Lck;proto-oncogene tyrosine-protein kinase LCK;t cell-specific protein-tyrosine kinase;T-lymphocyte specific protein tyrosine kinase p56lck;tyrosine-protein kinase Lck;YT16." in publications.
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What is the shipping cost for "Lck Antibody (OAAF00787)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Lck Antibody (OAAF00787)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Lck Antibody (OAAF00787)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "58 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Lck Antibody (OAAF00787)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "LCK"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "LCK"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "LCK"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "LCK"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "LCK"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "LCK"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.