KRT15 Antibody (OAGA01330)

Catalog No: OAGA01330

Size:100 ul

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
OAGA01330 diluted at 1:1000

Cytokeratin 15 antibody detects KRT15 protein at cytosol and membrane on HSC-3 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HSC-3 xenograft.
Cytokeratin 15 antibody (OAGA01330) dilution: 1:500.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cytokeratin 15. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.64mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: KRT15
• Gene Full Name: keratin 15, type I
• Alias Symbols: K15, CK15, K1CO
• NCBI Gene Id: 3866
• Description of Target: The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2.
• Uniprot ID: P19012