KARS ELISA Kit (Human) (OKCD00889)

Catalog No: OKCD00889 (Formerly GWB-KBBDN4)

Size:96WELLS

Human KARS ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	92-105%	91-101%	85-101%	84-91%
  EDTA plasma(n=5)	91-99%	89-103%	93-105%	84-98%
  heparin plasma(n=5)	89-103%	83-101%	94-105%	94-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl tRNA Synthetase (KARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl tRNA Synthetase (KARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl tRNA Synthetase (KARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lysyl tRNA Synthetase (KARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	91-105	99
  EDTA plasma(n=5)	80-91	86
  heparin plasma(n=5)	83-101	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-KARS Microplate: 96 Wells (12 x 8 Well Strips)
  • KARS Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated KARS Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.117 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lysyl tRNA Synthetase (KARS). No significant cross-reactivity or interference between Lysyl tRNA Synthetase (KARS) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: KARS1
• Gene Full Name: lysyl-tRNA synthetase 1
• Alias Symbols: CMTRIB;DEAPLE;DFNB89;KARS;KARS2;KRS;LEPID;lysine tRNA ligase;lysine--tRNA ligase;lysRS;Lysyl-tRNA synthetase.
• NCBI Gene Id: 3735
• Protein Name: Lysine--tRNA ligase
• Description of Target: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739).
• Uniprot ID: Q15046
• Protein Accession #: NP_001123561
• Nucleotide Accession #: NM_001130089.1
• Protein Size (# AA): 597