IRF3 Antibody (Phospho-Ser396) (OABF01188)

Catalog No: OABF01188

Size:100UL

Formalin-fixed and paraffin embedded mouse stomach tissue labeled with Anti-Phospho-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (OABF01188) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded rat colitis labeled with Anti-Phospho-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (OABF01188) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37C.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Monkey|Mouse|Porcine|Rat|Sheep
• Product Format: Liquid. Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: KLH conjugated synthetic phosphopeptide derived from human IRF3 around the phosphorylation site of Ser396
• Purification: Protein A Purified
• Concentration: 1 ug/ul
• Intended Use: For research use only. Not intended for diagnostic or therapeutic use.
• Application Info: WB: 1:300~1000
  IHC-P: 1:200~400
  Fluorescent-IHC: 1:50~200
  Fluorescent-ICC: 1:50~200

Target Info

• Gene Symbol: IRF3
• Gene Full Name: interferon regulatory factor 3
• Alias Symbols: IIAE7;interferon regulatory factor 3.
• NCBI Gene Id: 3661
• Protein Name: Interferon regulatory factor 3
• Description of Target: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
• Uniprot ID: Q14653