ILF3 ELISA Kit (Human) (OKEH05183)

Catalog No: OKEH05183

Size:96T

Human ILF3 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology ILF3 ELISA Kit (Human) (OKEH05183) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ILF3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ILF3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ILF3 captured in the well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 99%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <5.2%, n = 20
  Mean Inter-Assay Precision - <10.5%, n = 20
• ELISA Kit Component:
  • ILF3 Microplate: 96 Wells (12 x 8 Well strips)
  • ILF3 Lyophilized Standard: 2
  • 100X Biotinylated ILF3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 7.9 pg/mL
• Specificity: Natural and recombinant Human Interleukin enhancer-binding factor 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ILF3
• Gene Full Name: interleukin enhancer binding factor 3
• Alias Symbols: CBTF;Double-stranded RNA-binding protein 76;double-stranded RNA-binding protein, 76 kD;DRBF;DRBP76;dsRNA binding protein NFAR-2/MPP4;interleukin enhancer binding factor 3, 90kD;interleukin enhancer binding factor 3, 90kDa;interleukin enhancer-binding factor 3;M phase phosphoprotein 4, nuclear factor associated with DS RNA;MMP4;M-phase phosphoprotein 4;MPHOSPH4;MPP4;MPP4110;NF110;NF110b;NF90;NF90a;NF90b;NF90c;NF90ctv;NFAR;NFAR-1;NFAR110;NFAR2;NFAR-2;NFAR90;NF-AT-90;nuclear factor associated with dsRNA;nuclear factor of activated T-cells 110 kDa;nuclear factor of activated T-cells 90 kDa;nuclear factor of activated T-cells, 90 kD;TCP110;TCP80;translational control protein 80.
• NCBI Gene Id: 3609
• Protein Name: Q12906
• Description of Target: Interleukin enhancer-binding factor 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q12906
• Protein Accession #: NP_001131145.1
• Nucleotide Accession #: NM_001137673.1
• Protein Size (# AA): 894