ILF3 Antibody (OACA06969)

Catalog No: OACA06969

Immunohistochemistry of paraffin-embedded human cervical cancer at dilution 1:100

Immunohistochemistry of paraffin-embedded human kidney at dilution 1:100

All lanes: Interleukin enhancer-binding factor 3 antibody at 3ug/ml+HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 96,77,83,76,75 kDa
Observed band size: 95 kDa

All lanes: Interleukin enhancer-binding factor 3 antibody at 3ug/ml+HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 96,77,83,76,75 kDa
Observed band size: 95 kDa

Immunohistochemistry of paraffin-embedded human cervical cancer at dilution 1:100

Immunohistochemistry of paraffin-embedded human kidney at dilution 1:100

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Interleukin enhancer-binding factor 3 protein (785-894AA)
• Purification: Affinity Purified
• Preservative: 0.02% sodium azide
• Formulation: PBS with, 50% glycerol, pH7.3.
• Reference: [1]"Alternative splicing in the human interleukin enhancer binding factor 3 (ILF3) gene." Duchange N., Pidoux J., Camus E., Sauvaget D.Gene 261:345-353(2000). [2]"Characterization of two evolutionarily conserved, alternatively spliced nuclear phosphoproteins, NFAR-1 and -2, that function in mRNA processing and interact with the double-stranded RNA-dependent protein kinase, PKR."Saunders L.R., Perkins D.J., Balachandran S., Michaels R., Ford R., Mayeda A., Barber G.N.J. Biol. Chem. 276:32300-32312(2001). [3]"Functional proteomic analysis of human nucleolus."Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.Mol. Biol. Cell 13:4100-4109(2002).

Target Info

• Gene Symbol: ILF3
• Gene Full Name: interleukin enhancer binding factor 3
• Alias Symbols: CBTF;Double-stranded RNA-binding protein 76;double-stranded RNA-binding protein, 76 kD;DRBF;DRBP76;dsRNA binding protein NFAR-2/MPP4;interleukin enhancer binding factor 3, 90kD;interleukin enhancer binding factor 3, 90kDa;interleukin enhancer-binding factor 3;M phase phosphoprotein 4, nuclear factor associated with DS RNA;MMP4;M-phase phosphoprotein 4;MPHOSPH4;MPP4;MPP4110;NF110;NF110b;NF90;NF90a;NF90b;NF90c;NF90ctv;NFAR;NFAR-1;NFAR110;NFAR2;NFAR-2;NFAR90;NF-AT-90;nuclear factor associated with dsRNA;nuclear factor of activated T-cells 110 kDa;nuclear factor of activated T-cells 90 kDa;nuclear factor of activated T-cells, 90 kD;TCP110;TCP80;translational control protein 80.
• NCBI Gene Id: 3609
• Protein Name: Interleukin enhancer-binding factor 3
• Description of Target: RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed:28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed:21123651). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed:21123651, PubMed:28625552).
• Uniprot ID: Q12906
• Protein Accession #: NP_001131145
• Nucleotide Accession #: NM_001137673