Il17f Antibody: HRP Conjugated (OARA02291)

Catalog No: OARA02291

Size:100UG

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Lyophilized
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: HRP
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Additional Information: Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  Color: Substrate dependent
  Conjugation Reference: Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
  Preservative: 0.01% (w/v) Gentamicin Sulfate (Do NOT add Sodium Azide!)
  Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Reconstitution and Storage: 2°C to 8°C|-20°C or -80°C
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL17-F protein.
• Predicted Homology Based on Immunogen Sequence: Mouse; Rat
• Concentration: 1 mg/ml
• Specificity: This purified antibody has been heated to 56C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse IL17-F in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
• Application Info: This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 17.9 kDa in size corresponding to mouse IL17-F protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
  
  ELISA: 1:10,000-1:50,000
  WB: 1:1,000-1:5,000
  
• Reference: Gomez-Rodriguez,J., Sahu,N., Handon,R., Davidson,T.S., Anderson,S.M., Kirby,M.R., August,A. and Schwartzberg,P.L. (2009) Differential expression of interleukin-17A and -17F is coupled to T cell receptor signaling via inducible T cell kinase. Immunity 31 (4), 587-597.
  Nowak,E.C., Weaver,C.T., Turner,H., Begum-Haque,S., Becher,B., Schreiner,B., Coyle,A.J., Kasper,L.H. and Noelle,R.J. (2009) IL-9 as a mediator of Th17-driven inflammatory disease. J. Exp. Med. 206 (8), 1653-1660.
  Yu,Y., Iclozan,C., Yamazaki,T., Yang,X., Anasetti,C., Dong,C. and Yu,X.Z. (2009) Abundant c-Fas-associated death domain-like interleukin-1-converting enzyme inhibitory protein expression determines resistance of T helper 17 cells to activation-induced cell death. Blood 114 (5), 1026-1028. (2009)
  Hymowitz,S.G., Filvaroff,E.H., Yin,J.P., Lee,J., Cai,L., Risser,P., Maruoka,M., Mao,W., Foster,J., Kelley,R.F., Pan,G., Gurney,A.L., de Vos,A.M. and Starovasnik,M.A. (2001) IL-17s adopt a cystine knot fold: structure and activity of a novel cytokine, IL-17F, and implications for receptor binding. EMBO J. 20 (19), 5332-5341.

Target Info

• Gene Symbol: Il17f
• Gene Full Name: interleukin 17F
• Alias Symbols: C87042;IL-17F;interleukin-17F.
• NCBI Gene Id: 257630
• Protein Name: Interleukin-17F|interleukin-17F precursor [Mus musculus]
• Description of Target: Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (By similarity). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover. Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (By similarity). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation (PubMed:15477493).
• Uniprot ID: Q7TNI7
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_665855.2